Expression of Survivin in the Formalin-Fixed, Paraffin-embedded Specimens of Human Osteosarcoma May Be Used as a Diagnostic Marker

Document Type : Research Article

Authors

1 Department of Animal Biology, School of Natural Sciences, University of Tabriz, Tabriz-Iran

2 College of Pharmacy, Hawler Medical University, Erbil, Iraq

Abstract

Osteosarcoma is the most frequent malignant bone tumor with a peak incidence in the second and third decades of life. Diagnosis of patients with osteosarcoma is limited to clinico-pathological parameters whereas molecular markers of tumor initiation and promotion have not yet been identified. We aimed to study the expression of the anti-apoptotic survivin gene in osteosarcoma specimens. A total of 35 formalin-fixed, paraffin-embedded (FFPE) specimens including 25 tumoral and 20 non-tumoral paired-margins were collected. The expression of survivin was evaluated by employing real-time PCR and immunohistochemistry. Our data showed that survivin is differently expressed in osteosarcoma samples rather than non-tumoral margins. Real-time PCR showed that survivin is up-regulated in osteosarcoma specimens compared to margins (p <0.01). Furthermore, IHC confirmed that the survivin protein is significantly found in malignant parts of paraffin blocks. Our data show that survivin expression is modulated in osteosarcoma and may be considered as a diagnostic marker for further studies. Our data showed that survivin is differently expressed in osteosarcoma samples rather than non-tumoral margins. Real-time PCR showed that survivin is up-regulated in osteosarcoma specimens compared to margins (p <0.01). Furthermore, IHC confirmed that the survivin protein is significantly found in malignant parts of paraffin blocks. Our data show that survivin expression is modulated in osteosarcoma and could be considered as a diagnostic marker for further studies.

Keywords

References

Ambrosini G, Adida C, Altieri DC. 1997. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nat Med 3(8): 917-21.
Babaei E, Mowla SJ, Torbaghan S, Emadi Bayegi M. 2006. Detection of Survivin gene expression in formalin-fixed paraffin-embedded tissues of human osteosarcoma: its potential usefulness in diagnosis and prognosis of bone tumors.  Iran Biomed J 10 (1): 39-45.
Cao K, Tait SWG. 2018. Apoptosis and cancer: force awakens, phantom menace, or both? Int Rev Cell Mol Biol 337: 135-152.
Guindalini R, Mathias Machado MC, Garicochea B. 2013. Monitoring survivin expression in cancer: implications for prognosis and therapy. Mol Diagn Ther 17 (6): 331-42.
Jaiswal PK, Goel A, Mittal RD. 2015. Survivin: a molecular biomarker in cancer. Indian J Med Res 141(4): 389-97.
Jannat Alipoor F, Asadi M, Torkzadeh-Mahani M. (2017). LncRNA miat promotes proliferation of cervical cancer cells and acts as an anti-apoptotic factor, J Genet Resour 3(2): 80-86.
Kyani K, Babaei E, Hosseinpour Feizi MA, Vandghanooni S, Montazeri V, Halimi M. 2014. Detection of survivin 2α gene expression in thyroid nodules. J Cancer Res Ther 10(2): 312-20.
Lau CC. 2009. Molecular classification of osteosarcoma. Cancer Treat Res 152: 459-65
Liu G, Pei F, Yang F, Li L, Amin AD, Liu S, Buchan JR, Cho WC. 2017. Role of autophagy and apoptosis in non-small-cell lung cancer. Int J Mol Sci 10: 18(2). doi: 10.3390/ijms18020367.
Moore DD, Luu HH. 2014. Osteosarcoma. Cancer Treat Res 162: 65-92.
Nie T, Daim, Zong S. 2005. Expression of survivin gene and its relationship with clinical multidrug resistance in osteosarcoma. Chin Ger J Clin Oncol 4: 164-166.
Otoukesh B, Boddouhi B, Moghtadaei M, Kaghazian P, Kaghazian M. 2018. Novel molecular insights and new therapeutic strategies in osteosarcoma. Cancer Cell Int 18: 158. doi: 10.1186/s12935-018-0654-4
Saraf AJ, Fenger JM, Roberts RD. 2018. Osteosarcoma: accelerating progress makes for a hopeful future. Front Oncol 8(4): 1-7.
Varshney J, Scott MC, Largaespada DA, Subramanian S. 2016. Understanding the osteosarcoma pathobiology: a comparative oncology approach. Vet Sci 18: 3(1). doi: 10.3390/vetsci3010003.
Zamborsky R, Kokavec M, Harsanyi S, Danisovic L. 2019. Identification of prognostic and predictive osteosarcoma biomarkers. Med Sci (Basel) 11: 7(2). doi: 10.3390/medsci7020028.
Zambrano J, Yeh ES. 2016. Autophagy and apoptotic crosstalk: mechanism of therapeutic resistance in HER2-positive breast cancer. Breast Cancer (Auckl) 13(10): 13-23.
Zou J, Gan M, Mao N, Zhu X, Shi Q, Yang H. 2010. Sensitization of osteosarcoma cell line SaOS-2 to chemotherapy by downregulating survivin. Arch Med Res. 41(3): 162-169.
Journal of Genetic Resources
Volume 6, Issue 1
February 2020
Pages 49-53

Files

Share

How to cite

Statistics