Galectin 1 and Superoxide Dismutase are Involved in Wound Healing by Larval Therapy

Document Type: Research Article

Authors

1 Department of Biology, Faculty of Sciences, Hakim Sabzevari University, Sabzevar, Iran

2 Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

10.22080/jgr.2019.2417

Abstract

Galectin-1 and superoxide dismutase are two known molecules in the wound healing process that induce such healing by different mechanisms in the wound site. Larval therapy is one of the methods use by Lucilia sericata fly larvae, nowadays returned to the list of therapeutic methods despite chronic diabetic ulcers and antibiotic resistance of bacteria. In this study, we aimed to evaluate the effect of larvae extract on fibroblast cells to determine its role in the levels of Galectin-1 and superoxide dismutase proteins in fibroblast cells. To determine proteomic changes, 3T3 fibroblast cells were treated with larval extract, 3T3 fibroblast cells were cultured and divided into two groups after appropriate density. The first group was considered as control and the second group was treated with larvae extract at a concentration of 12.5 μg/ml. After 24 hours, the two-dimensional gel electrophoresis method for protein level and real-time PCR for gene expression studies were used. In 2D gel testing, three spots were successfully identified including galectin-1, superoxide dismutase, and glyceraldehyde-3-phosphate dehydrogenase. The expression of these three proteins was significantly increased in the cells treated with larvae extract compared to the control cells. Also, the quantitative expression of these genes was confirmed by real-time PCR. Finally, it was found that the treatment of fibroblast cells with larvae extract increased expression of galectin-1, superoxide dismutase and glyceraldehyde-3-phosphate dehydrogenase, which their positive effect on wound healing is well known.

Keywords


Myers BA. 2008. Wound management: principles and practice. New York: Pearson/Prentice Hall

Rezaie F, Momeni-Moghaddam M, Naderi-Meshkin H. 2019. Regeneration and repair of skin wounds: various strategies for treatment. Int J Low Extrem Wounds 18(3): 247-261.

Schultz GS, Sibbald RG, Falanga V, Ayello EA, Dowsett C, Harding K. 2003. Wound bed preparation: a systematic approach to wound management. Wound Repair Regen 11: 1-28.

Sanjari T, Momeni-Moghaddam M, Vatandoost J, Hajjar T. 2013. Mouse skin wound healing using Lucilia sericata maggot extract. J Cell Mol Res 30(1): 26-39.

Sherman RA. 2002 . Maggot therapy for foot and leg wounds. Int J Low Extrem Wounds 1(2): 135-142.

Davydov L. 2011. Maggot therapy in wound management in modern era and a review of published literature. J Pharm Pract 24(1): 89-93.

Church J. 1999. Larva therapy in modern wound care: a review. Primary Intent 7: 63-68.

Kaihanfar M, Momeni-Moghaddam M, Moghaddam MJM, Hajar T, Pak VD, Bidi JO. 2018. Investigation of antimicrobial effects of treated Lucilia sericata larvae extract on bacteria. Iran J  Microb 10(6): 409-416.

Boateng JS, Matthews KH, Stevens HN, Eccleston GM. 2008. Wound healing dressings and drug delivery systems: a review. J Pharm Sci 97(8): 2892-2923.

Daroqui CM, Ilarregui JM, Rubinstein N, Salatino M, Toscano MA, Vazquez P, et al. 2007. Regulation of galectin-1 expression by transforming growth factor β1 in metastatic mammary adenocarcinoma cells: implications for tumor-immune escape. Cancer Immunol Immunother56(4): 491-499.

Steiling H, Munz B, Werner S, Brauchle M. 1999. Different types of ROS-scavenging enzymes are expressed during cutaneous wound repair. Exp Cell Res247(2): 484-494.

Vorauer-Uhl K, Fürnschlief E, Wagner A, Ferko B, Katinger H. 2001. Topically applied liposome encapsulated superoxide dismutase reduces postburn wound size and edema formation. Eur J Pharm Sci14(1): 63-67.

Marrotte EJ, Chen DD, Hakim JS, Chen AF. 2010. Manganese superoxide dismutase expression in endothelial progenitor cells accelerates wound healing in diabetic mice. J Clin Invest120(12): 4207-4219.

Lim MJ, Ahn J, Yi JY, Kim MH, Son AR, Lim DS, et al. 2014. Induction of galectin-1 by TGF-β1 accelerates fibrosis through enhancing nuclear retention of Smad2. Exp Cell Res326(1): 125-135.

Lin YT, Chen JS, Wu MH, Hsieh IS, Liang CH, Hsu CL, et al. 2015. Galectin-1 accelerates wound healing by regulating the neuropilin-1/Smad3/NOX4 pathway and ROS production in myofibroblasts. J Investig Dermatol135(1): 258-268.

Daeschlein G, Mumcuoglu K, Assadian O, Hoffmeister B, Kramer A. 2007. In vitro antibacterial activity of Lucilia sericata maggot secretions. Skin Pharmacol Physiol 20(2): 112-5.

Jaklič D, Lapanje A, Zupančič K, Smrke D, Gunde-Cimerman N. 2008. Selective antimicrobial activity of maggots against pathogenic bacteria. J Med Microbiol 57(5): 617-625.

Sirover MA. 1999. New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta 1432(2): 159-184.

Sirover MA. 2011. On the functional diversity of glyceraldehyde-3-phosphate dehydrogenase: biochemical mechanisms and regulatory control. Biochim Biophys Acta  1810(8): 741-751.

Foss DL, Baarsch MJ, Murtaugh MP. 1998. Regulation of hypoxanthine phosphoribosyltransferase, glyceraldehyde‐3‐phosphate dehydrogenase and β‐actin mRNA expression in porcine immune cells and tissues. Anim Biotechnol 9(1): 67-78.

Berry MD, Boulton AA. 2000. Glyceraldehyde‐3‐phosphate dehydrogenase and apoptosis. J Neurosci Res 60(2): 150-154.

Camby I, Le Mercier M, Lefranc F, Kiss R. 2006. Galectin-1: a small protein with major functions. Glycobiology 16(11):137-157.