Investigating the Distribution of ERCC2 (rs13181) Gene Polymorphism in Gastric Cancer Patients in Mazandaran: A Case-control Study

Document Type : Research Article


1 Pharmacutical Research Center,Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

2 Pharmaceutical Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

3 Genetics Department of Sana Non-Profit University, Sari, Iran


Background and objectives: Gastric cancer is one of the most common cancers of the gastrointestinal tract in the world, which also has a high prevalence in Iran. ERCC2 gene is considered one of the major genes related to gastric cancer. The present study aimed to investigate the relationship between rs13181 polymorphism of ERCC2 gene. The polymorphism in the promoter region of ERCC2 gene can affect the activity of this gene and thus the susceptibility to gastric cancer.
Materials and Methods: In this case-control study, 81 patients and 75 healthy individuals were recruited. Five cc peripheral blood of individuals was collected in EDTA-containing tubes. Genomic DNA was extracted using the Salting-out method and polymorphism was genotyped using the PCR-RFLP method.
Results: The prevalence of rs13181 (G > T )genotype and polymorphism allele were significantly different between patients with gastric cancer and control group (both p Conclusion: Our results confirmed the association between rs13181 polymorphism of ERCC2 gene and the increased risk of gastric cancer in Mazandaran province.


Berwick M, Vineis P. 2000. Markers of DNA repair and susceptibility to cancer in humans: an epidemiologic review. J Natl Cancer Inst 92: 874-897.
Chu H, Gu D, Xu M, Xu Z, Gong W, Tan Y, Zhou J, Tong N, Zhang Z, Chen J, Wang M. 2013. A genetic variant in ERCC2 is associated with gastric cancer prognosis in a Chinese population. Mutagenesis 28(4):441-446.
Colagar AH, Chaichi MJ, Khajvand T. 2011. Fourier transform infrared microspectroscopy as a diagnostic tool for distinguishing between normal and malignant human gastric tissue. J Biosci 36(4):669-677
Damandan M; Moradpour Hesari R. 2016. The association of pre-mir-196a2 T/C polymorphism and risk of gastric cancer in Ardabil, Iran. J Genet Resour 2(1):48-51.
de Boer JG. 2002. Polymorphisms in DNA repair and environmental interactions. Mut Res 509: 201-210.
Debniak T, Scott RJ, Huzarski T, Byrski T, Masojc B, van de Wetering T, et al. 2006. XPD common variants and their association with melanoma and breast cancer risk. Breast Cancer Res Treat 98: 209-215.
Dicken BJ, Bigam DL, Cass C, Mackey JR, Joy AA, Hamilton SM. 2005. Gastric adenocarcinoma: review and considerations for fature direction. Ann Surg 241(1):27-39.
Dragovich T, Campen C. 2009. Anti-EGFR-targeted therapy for esophageal and gastric cancers: An evolving concept. J Oncol 2009: 804108.
Hengartner M. 2000. The biochemistry of apoptosis. Nature 407:770-776.
Kietthubthew S, Sriplung H, Au WW, Ishida T. 2006. Polymorphism in DNA repair genes and oral squamous cell carcinoma in Thailand. Int J Hyg Environ Health 209: 21-29.
Li YL, Tian Z, Zhang JB, Fu BY. 2012. CDH1 promoter polymorphism and stomach cancer susceptibility. Mol Biol Rep 39(2):1283-1286.
Li Zhang, Hua Wang, Zhang-Jun Song, Xiao-Zhi Zhang. 2017. Impact of single nucleotide polymorphisms in ERCC2 gene and their interaction with smoking on esophageal squamous cell carcinoma risk in Chinese Han population. Int J Clin Exp Pathol 10(1): 730-735.
Liu ZF, Aikenmu ALJ, Zhao J, Meng QC, Fang R. 2015. Influence of ERCC2 gene Polymorphisms on the Treatment outcome of osteosarcoma. Genet Mol Res 14(4):12967-12972.
Majumder M, Sikdar N, Ghosh S, Roy B. 2007. Polymorphisms at XPD and XRCC1 DNA repair loci and increased risk of oral leukoplakia and cancer among NAT2 slow acetylators. Int J Cancer 120: 2148-2156.
Mir Mohammadrezaei F. 2015. The Role of chk2 in Response to DNA Damage in Cancer Cells. J Genet Resour 1(1): 31-34.
Monteiro MS, Boas DBV, Gigliotti CB, Salvadori DM. 2014. Association among XRCC1, XRCC2, and BLHX gene polymorphisms and chromosome instability in lymphocytes from patients with endometriosis and ovarian cancer. Genet Mol Res 13: 636-648.
Neumann AS, Sturgis EM, Wei Q. 2005. Nucleotide excision repair as a marker for susceptibility to tobacco-related cancers: a review of molecular epidemiological studies. Mol Carcinog 42: 65-92.
Oshima N, Yamada Y, Nagayamas S, Kawada K, Hasegawa S, Okabe H, et al. 2014. Induction of cancer stem cell properties in colon cancer cells by defined factors. Plos One 9(7): e101735.
Siadati S, Jalali Nadoushan M, Davati A, Torabi Parizi G, Ghasemi S. 2012. Study of the Murine Double Minute 2 status in patients with gastric and colorectal carcinomas and its correlation with prognostic factors. Indian J Pathol Microbiol 55(2):192-5.
Sileng A, Pan R, Li G, Wei W, li J, Zhang M, Li M, Zhang Z, Lin J, Liao C. 2016. ERCC1 rs3212986 and ERCC2 rs13181 gene polymorphisms contributes to the susceptibility to pancreatic cancer in a Chinese population. Int J Clin EXP Pathol 9(5): 5687-5693.
Song YZ, Duan MN, Zhang YY, Shi WY, Xia CC, Dong LH. 2015. ERCC2 Polymorphisms and radiation-induced adverse effects on normal tissue: systematic review with meta-analysis and trial sequential analysis. Radiat Oncol 10:247.
Shokrzadeh M, Fattahi I, Mohammadpour A, Mashhadban AH. 2017. Presence of CagA gene and its antibiotic resistance pattern in Helicobacter pylori isolates.   J Mazandaran Univ Med Sci 27(154): 60-72.
Shokrzadeh M, Rahbari Jeyd P, Mohammadpour A, Zaboli F, Mohammadnejad FZ, Ghaffari Charati M, Saleh Tabari Y. 2017. Frequency of exoT and exoS Genes among Pseudomonas Aeruginosa Isolates and Antibiotic Resistance in Burn Patients in Sari Zare Hospital, Iran. J Mazandaran Univ Med Sci 27(154): 51-59.
Tafrihi M, Toosi S, Minaei T, Gohari AR, Niknam V, Arab Najafi SM. 2014. Anticancer properties of Teucrium persicum in PC-3 prostate cancer cells. Asia Pac J Cancer Prevent 15(2): 785-791.
Tambini CE, Spink KG, Ross CJ, Hill MA, Thacker J. 2010. The importance of XRCC2 in RAD51-related DNA damage repair. DNA Repair 9: 517-525.
Thacker J. 1999. A surfeit of RAD51-like gene? Trends Genet 15: 166-168.
Thacker J. 2005. The RAD51 gene family, genetic instability and cancer. Cancer Lett 219: 125-135.
Wu Q, Christensen LA, Legerski RJ, Vasquez KM. 2005. Mismatch repair participates in error-free processing of DNA interstrand crosslinks in human cells. EMBO Rep 6: 551-557.
Zhang Q, Lv LY, Li BJ, Zhang J and Wei F. 2015. Investigation of ERCC1 and ERCC2 gene polymorphisms and response to chemotherapy and overall survival in osteosarcoma. Genet Mol Res 14(3): 11235-11241.
Zhao R, Ying MF. 2015. Association between ERCC1 and ERCC2 Polymorphisms and breast cancer risk in a Chinese population. Genet Mol Res 15(1): gmr.15017263.
Zheng DL, Tang GD, Chen YN, Zhang T, Qin MB. 2016. Genetic variability of ERCC1 and ERCC2 genes involved in the nucleotide excision repair pathway influences the treatment outcome of gastric cancer. Genet Mol Res 15(2): gmr.15027384.
Zheng K, He M.G, Tan D and Wang Z.X. 2015. Assciation between ERCC1 and ERCC2 gene polymorphisms and susceptibility to pancreatic cancer. Genet Mol Res 15(1): gmr. 15017879.