Investigating the Distribution of ERCC2 (rs13181) Gene Polymorphism in Gastric Cancer Patients in Mazandaran: A Case-control Study

Document Type: Research Article

Authors

1 Pharmacutical Research Center,Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

2 Pharmaceutical Research Center, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

3 Genetics Department of Sana Non-Profit University, Sari, Iran

Abstract

Background and objectives: Gastric cancer is one of the most common cancers of the gastrointestinal tract in the world, which also has a high prevalence in Iran. ERCC2 gene is considered one of the major genes related to gastric cancer. The present study aimed to investigate the relationship between rs13181 polymorphism of ERCC2 gene. The polymorphism in the promoter region of ERCC2 gene can affect the activity of this gene and thus the susceptibility to gastric cancer.
Materials and Methods: In this case-control study, 81 patients and 75 healthy individuals were recruited. Five cc peripheral blood of individuals was collected in EDTA-containing tubes. Genomic DNA was extracted using the Salting-out method and polymorphism was genotyped using the PCR-RFLP method.
Results: The prevalence of rs13181 (G > T )genotype and polymorphism allele were significantly different between patients with gastric cancer and control group (both p Conclusion: Our results confirmed the association between rs13181 polymorphism of ERCC2 gene and the increased risk of gastric cancer in Mazandaran province.

Keywords


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