Platycladus orientalis Extracts with Antioxidant Activity from North of Iran

Document Type: Research Article


1 Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.

2 Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.


Recently, the formation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been implicated in the oxidative deterioration of food products as well as in the pathogenesis of several human diseases such atherosclerosis, diabetes mellitus, chronic inflammation, neurodegenerative disorders and certain types of cancer. Plant secondary metabolites mainly phenolics and flavonoids are commonly found in various fruits, vegetables and herbs. The plant-derived antioxidants may use as therapeutics for before mentioned diseases. They play this role through acting as reducing agents by donating hydrogen, acting as chelators, quenching singlet oxygen and scavenging free radicals. In the present study, four extracts of Platycladus orientalis (L.) Franco leaves by maceration in water, methanol, ethanol and ethyl acetate, were prepared and the antioxidant activities of the extracts have been investigated by DPPH method, H2O2 scavenging activity and reducing power assays. The total phenol and flavonoid contents of these extracts were determined and compared with their antioxidant potential. The results showed that antioxidant effect of these extracts is increased in the following order: water < ethyl-acetate < ethanol< methanol extract. Among the extracts, the methanol extract showed as strong extract in both DPPH radical (IC50: 11.45 µg/ml) and H2O2 (IC50: 49.9 µg/ml) scavenging activity assays. The ethanol extract was shown a highest reductive potential in comparison with other extracts. There was a positive and significant correlation between antioxidant properties and total phenol and flavonoid content of P. orientalis. It was concluded that the extracts of P. orientalis can use as a source of natural antioxidants in prevention of cellular oxidation.


Anderson RF, Fisher LJ, Hara Y, Harris T, Mak B, Melton LD, Packer JE. 2001. Green tea catechins partially protect DNA from OH radical-induced strand breaks and base damage through fast chemical repair of DNA radicals. Carcinogenesis 22: 1189-1193.

Antolovich M, Prenzler P, Robards K, Ryan D. 2000. Sample preparation in the analysis of phenolic compounds in fruits. Analyst 125: 989-1009.

Asili J, Asghari G, Sadat Ebrahimi SE, Jaroszewski JW. 2007. Influence of extraction methods on the yield and chemical composition of essential oil of Platycladus orientalis (L.) Franco. Jundishapur J Nat Pharm Prod 2: 25-33.

Blois MS. 1958. Antioxidants determination by the use of a stable free radical. Nature 4617: 1199-1200.

Cheng JC, Fang JG, Chen WF, Zhou B, Yang L, Liu ZL. 2006. Structure–activity relationship studies of resveratrol and its analogues by the reaction kinetics of low density lipoprotein peroxidation. Bioorg Chem 34: 142-157.

Choi Y, Moon A, Kim YC. 2008. A pinusolide derivative, 15-methoxypinusolidic acid from Biota orientalis inhibits inducible nitric oxide synthase in microglial cells: implication for a potential anti-inflammatory effect. Int Immunopharmacol 8: 548-555.

Cook NC, Samman S. 1996. Flavonoids-chemistry, metabolism, cardioprotective effects and dietary sources. Nutr Biochem 7: 66-76.

Diplock AT. 1997. Will the Good Fairies please prove to us that vitamin E lessens human degenerative disease? Free Radic Res 27: 522-532.

Emami S A, Asili J, Mohagheghi Z, Hassanzadeh MK. 2007. Antioxidant activity of leaves and fruits of Iranian conifers. Evid Based Alternat Med Complement 4:313-319.

Han BH, Yang HO, Kang YH, Suh DY, Go HJ, Song WJ, Kim YC, Park MK. 1998. In vitro platelet-activating factor receptor binding inhibitory activity of pinusolide derivatives: a structure-activity study. J Med Chemo 41: 2626-2630.

Hashemi SM, Safavi S A. 2012. Chemical constituents and toxicity of essential oils of oriental arborvitae, Platycladus orientalis (L.) Franco, against three stored-product beetles.  Chil J Agr Res 72:188-194.

Liyana-Pathirana CM, Shahidi F. 2007. Antioxidant and free radical scavenging activities of whole wheat and milling fractions. Food Chem 101: 1151-1157.

Matkowski A, Blume Y, Smertenko P, Durzan DJ. 2006. Plant phenolic metabolites as antioxidants and antimutagens.  NATO Life Science Monographs 376: 129-148.

Mier S, Kanner J, Akiri B, Hadas SP. 1995. J Agric Food Chem 43: 1813-1815.

Nabavi SM, Nabavi SF, Alinezhad H, Zare M and Azimi R. 2012. Biological activities of flavonoid-rich fraction of Eryngium caucasicum Trautv. Eur Rev Med Pharmacol Sci16: 81-87.

Oyaizu M. 1986. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jap J Nutr 44: 307-315.

Ruch RJ, Cheng ST, Klauring JE. 1989. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 10: 1003-1008.

Shahidi F, Wanasundara PKJPD. 1992. Phenolic antioxidants. Crit Rev Food Sci Nutr 32: 67-103.

Singelton VR, Orthifer R, Lamuela-Raventos RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol 299: 152-178.

Srivastava P, Kumar P, Singh DK, Singh VK. 2012. Biological properties of Thuja orientalis Linn. Adv Life Sci 2: 17-20.

Tsuda H, Ohshima Y, Nomoto H, Fujita K, Matsuda E, Iigo M, Takasuka N, Moore MA. 2004. Cancer prevention by natural compounds. Drug Metab Pharmacokin 19: 245-263.

Wei QY, Chen WF, Zhou B, Yang L, Liu ZL. 2006. Inhibition of lipid peroxidation and protein oxidation in rat liver mitochondria by curcumin and its analogues. Biochim Biophys Ata 1760: 70-77.

Willet WC. 2002. Balancing life-style and genomics research for disease prevention. Science 296: 695-698.

Xu G, Ryoo I, Kim Y, Choo S, Yoo I. 2009. Free radical scavenging and antielastase activities of flavonoids from the fruits of Thuja orientalis. Arch Pharm Res 32: 275-282