Effects of Plant Growth Regulators and Explant on Callus Induction in Cuminum cymium L.

Document Type : Research Article


1 Department of Medicinal Plants, Institute of Higher Education, Jahad-e-Daneshgahi, Kermanshah Unit, Iran

2 Agronomy and Plant Breeding Department, Razi University, Kermanshah, Iran

3 Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran


Cumin (Cuminum cyminum L.) as a member of the Apiaceae family is one of the most important medicinal plants in Iran. The purpose of this study is to evaluate the effect of plant growth regulators and explant type on callus induction in cumin. For this purpose the cumin seeds (Kuhbanan accession) were disinfected with sodium hypochlorite and alcohol and cultured on MS basal medium. Leaf and hypocotyl explants were prepared from sterile seedlings and used to produce callus on MS medium containing 0.0, 0.5, 1.0 and 2.0 mg/l NAA with 0.0 and 0.5 mg/l BAP. The experimental was as completely randomized factorial design with three replications. The results of callus induction showed that the explants type, hormone, and their interactions have non-significant effects on the callus induction percentage. Also, explants showed significant effect on callus growth rate (CGR). However hormones and hormone- explant interactions did not have a significant effect on CGR. The results showed that the medium containing 1 mg/l NAA and 0.5 mg/l BAP was known as the best callus growth rate medium for cumin (0.238 mm/d). Comparing the mean interactions of the explants in hormone on CGR showed that 0.5 mg/l of NAA + 0.5 mg/l of BA in leaf explant has the highest effect (0.248 mm/d).


Ebrahimie E, Habashi AA, Ghareyazie B, Ghannadha M, Mohammadi M. 2003. A rapid and efficient method for regeneration of plantlets from embryo explants of cumin. Plant Cell Tissue Organ Cult 75: 19-25.
Ebrahimie E, Naghavi MR, Hosseinzadeh A, Behamta MR, Mohammadi-Dehcheshmeh M, Sarrafi A, Spangenberg G. 2007. Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (cuminum cyminum l.) as a model material. Plant Cell Tissue Organ Cult 90: 293–311.
Kahrizi D, Arminian A, Masumi Asl A. 2011. In vitro Plant Breeding. 2nd Edition.  Razi University Press, Kermanshah, Iran
Lawrence BM. 1995. Progress in essential oils. Perfum Flavor 20: 47–54.
Martin KP. 2004. Plant regeneration through somatic embryiogenesis in medicinally important Eryngium foetidum L. In Vitro Cell Dev Biol Plant 40: 459-463.
Masoumi S, Kahrizi D, Rostami-Ahmadvandi H, Soorni J, KianiS, Mostafaie A, Yari K. 2012. Genetic diversity study of some medicinal plant accessions belong to Apiaceae family based on seed storage proteins patterns. Mol Biol Rep 39: 10361-10365.
Moon HK, Stomp AM. 1997. Effects of medium components and light on callus induction, growth, and frond regeneration in Lemna gibba (Duckweed). In Vitro Cell Dev Biol Plant 33: 20-25.
Murashig ET, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497.
Sharifi M. 1995. Comparative investigation of essences of Cuminum cyminum and Cuminum cyminum seed and explant fragments. M.Sc. Thesis, Agricultural College, Tehran University. Tehran, Iran.
Soorni J, Kahriz, D, Molsaghi M. 2012. The Effects of Photoperiod and 2, 4-D Concentrations on Callus Induction of Cuminum cyminum's Leaf Explant: an Important Medicinal Plant. Asian J Biol Sci 5: 378-383.
Tawfik AA, Noga G. 2001. Adventitious shoot proliferation from hypocotyl and internodal stem explants of cumin. Plant Cell Tissue Organ Cult 66: 141–147.
Tawfik AA, Noga G. 2002. Cumin regeneration from seedling derived embryogenic callus in response to amended Kinetin. Plant Cell Tissue Organ Cult 69: 35-40.
Valizadeh M, Tabar SKK,  Nematzadeh GA. 2007. Effect of plant growth regulators on callus induction and regeneration of cumin (Cuminum cyminum). Asian J Agric Res 1: 17-22.