Taxonomic Relationships of Ten Fritillaria Species of Subgenera Fritillaria and Theresia Based on Analysis of Flower Qualitative and Quantitative Morphological Characters

Document Type: Research Article

Authors

Department of Biology, Faculty of Science, University of Shahrekord, Shahrekord, Iran

Abstract

Flower morphology of 10 Fritillaria species of subgenera Theresia and Fritillaria growing wild in Iran is studied using seventy morphological characters. Qualitative and quantitative datasets separated taxa into subgenera, sectional and groups. Three enigmatic species F. caucasica, F. zagrica (endemic to Zagros Mountains) and F. pinardii (recently reported from Zagros Mountains) appeared as closely related, distinct taxa. F. chlorantha (endemic to central Zagros Mountains) was put in an intermediate position between members of sections Trichostyleae and Olostyleae. Two distinct groups of taxa corresponding to Caucasian group and sect. Trichostyleae, were clustered in subgenus Fritillaria, mainly by three quantitative characters, i.e. lengths of the petal, sepal, and the stamen. Exploratory analyses of both quantitative and qualitative characters produced consistent results and showed the application of quantitative characters of flower in refining the taxonomy of this genus.

Keywords


Baker JG. 1874. A revision of the genera and species of Tulipeae. J Linn Soc Bot 14: 211-310.

Bakhshi-Khaniki G. 2002a. Giemsa C-banding survey and chromosome morphometry in some species of Rhinopetalum (Liliaceae). Bot Chronika 15: 51-62.

Bakhshi-Khaniki G. 2002b. Chromosome number of all Iranian species of Fritillaria caucasica group (Liliaceae). Nucleus 45: 103-108.

Bakhshi-Khaniki G. 2002c. Chromosome number of Fritillaria subgenera Petilium and Theresia (Liliaceae). Nucleus 45: 6-11.

Bakhshi-Khaniki G. 2005. Giemsa C-banding studies on interphase nuclei of Iranian species of Fritillaria and Rhinopetalum (Liliaceae). P Natl A Sci India B 75: 294.

Bennett MD, Leitch IJ. 2012. Plant DNA C-values database (release 6.0, December 2012). Available at: http://www.kew.org/cvalues/.

Celebi A, Teksen M, Acik L, Aytac Z. 2008. Taxonomic relationships in genus Fritillaria (Liliaceae): Evidence from RAPD-PCR and SDS-PAGE of seed proteins. Acta Bot Hung 50: 325-343.

Christensen K, Zarrei M, Kuzmina M, Talent N, Lin C, Dickinson TA. 2014. Crataegus × ninae-celottiae and C. × cogswellii (Rosaceae, Maleae), two spontaneously formed intersectional nothospecies. PhytoKeys 36: 1-26.

Day PD, Berger M, Hill L, Fay MF, Leitch AR, Leitch IJ, Kelly LJ. 2014. Evolutionary relationships in the medicinally important genus Fritillaria L. (Liliaceae). Mol Phylogen Evol 80: 11-19.

Huson DH, Bryant D. 2006. Application of Phylogenetic Networks in Evolutionary Studies. Mol Biol Evol 23: 254-267.

Jafari H, Babaei A, Karimzadeh G, Ahmadi-Roshan M. 2014. Cytogenetic study on some Fritillaria species of Iran. Plant Syst Evol 300: 1373-1383.

Karypis G. 2003. CLUTO: a clustering toolkit, Ver 2.1.1 Available at: http://glaros.dtc.umn.edu/gkhome/cluto/cluto/

Khourang M, Babaei A, Sefidkon F, Naghavi MR, Asgari D, Potter D. 2014. Phylogenetic relationship in Fritillaria spp. of Iran inferred from ribosomal ITS and chloroplast trnL-trnF sequence data. Biochem Syst Ecol 57: 451-457.

Metin O, Turktas M, Aslay M, Kaya E. 2013. Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP. ‎Turk J Biol 37: 273-279.

Ozhatay N. 2000. Fritillaria L. In Guner A, Ozhatay N, Ekim T, Baser KHC (Eds.). Flora of Turkey and the East Aegean Islands (suppl. 2). Vol. 11: 243-246. Edinburgh: Edinburgh University Press.

Rechinger KH. 1990. Flora Iranica: Liliaceae II. Akademische Druck-u. Verlagsanstalt, Graz.

Rix E. 1974. Notes on Fritillaria (Liliaceae) in the eastern Mediterranean region, I & II. Kew Bull 29: 633-654.

Rix EM, Frank E, Webster G. 2001. Fritillaria: a revised classification, together with an updated list of species. Fritillaria Group of Alpine Garden Soc., Edinburgh.

Rix EM. 1984. Fritillaria L. In: Davis PH (Ed.), Flora of Turkey and the East Aegean Islands. University Press, Edinburg, Vol. 8, pp. 284-302.

Rix EM. 1997. Fritillaria L. (Liliaceae) in Iran. Iran J Bot 1: 75–95.

Rohlf FJ. 2000. NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, Setauket, NY.

Ronsted N, Law S, Thornton H, Fay MF, Chase MW. 2005. Molecular phylogenetic evidence for the monophyly of Fritillaria and Lilium (Liliaceae; Liliales) and the infrageneric classification of Fritillaria. Mol Phylogen Evol 35: 509-527.

Schneider CA, Rasband WS, Eliceiri KW. 2012. NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9: 671-675.

Sharifi-Tehrani M, Advay M. 2015. Assessment of relationships between Iranian Fritillaria (Liliaceae) species using chloroplast trnH-psbA sequences and morphological characters. J Genet Resour 2: 89-100.

Sing-chi L, Yi-bo C. 1996. Critical notes on the genus Fritillaria L. (Liliaceae) of Xinjiang, China. Acta Phytotaxon Sin 34: 77-85.

Sneath PH. 1995. Thirty years of numerical taxonomy. Syst Biol 44: 281-298.

Teksen M, Aytac Z. 2011. The revision of the genus Fritillaria L. (Liliaceae) in the Mediterranean region (Turkey). Turk J Bot 35: 447-478.

Zhao Y, Karypis G. 2001. Criterion functions for document clustering: Experiments and analysis. Technical Report TR #01–40, Department of Computer Science, University of Minnesota, Minneapolis, MN.