Chromosome C-banding in Mus musculus L.1766 Strains Shows a Fixed Position for the Centromere and Variable Amounts in Different Populations

Document Type: Research Article


1 Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

2 Rodentology Research Department, Ferdowsi University of Mashhad, Mashhad, Iran

3 Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran


Three subspecies of Mus musculus have been recognized in Iran so far. The house mouse (genus0T 0T2TMus2T, species0T 0T2TMus musculus2T) are recognized for their highly conserved morphology and chromosomal structure, but some chromosomal characters offer accurate taxonomic markers in this species that has been shown any unambiguous diagnostic morphological traits. Among the chromosomal characters, centromeric heterochromatin is more useful to identify mouse subspecies and populations. 0T 0TIn this study, Samples were collected from 27 stations in Iran and study was performed by the centromeric heterochromatin banding (C-banding). Results indicated that all samples had 40 acrocentric chromosomes and all chromosomes had fixed position for the centromere. The strains had the same amount of C-banding material on homologous chromosomes but showed variation in the amount on different populations.


Bonhomme F, Rivals E, Orth A, Grant G, Jeffreys AJ, Rj Bois, P. 2007. Species-wide distribution of highly polymorphic minisatellite markers suggests past and present genetic exchanges among house mouse subspecies. Genome Biol 8: R80.

Boursot P, Bonhomme F, Catalan J, Moriwaki K. 1989. Variations of Y chromosome repeated sequence across subspecies of Mus musculus. Heredity 63:289-297.

Britten RJ, Kohne DE. 1968. Repeated DNA sequences in DNA. Science 161: 529-540.

Broccoli D, Miller OJ, Miller DA. 1990. Relationship of minor satellite to centromere activity. Cytogenet Cell Genet 54: 182-186.

Darvish J, Orth A, Bonhomme F. 2006. Genetic transition in the house mouse Mus musculus of eastern Iranian plateau. Folia Zool 55: 176-182.

Davidson MT. 1989. Centromeric chromosome variants. In: Lyon MF, Searle AG, eds. Genetic Variants and Strains of the Laboratory Mouse, Oxford University Press.

Dev VG, Grewal MS, Miller DA, Kouri RE, Hutton JJ, Miller OJ. 1971. The quinacrine fluorescence karyotype of Mus musculus and demonstration of strain differences in secondary constrictions. Cytogenet Genome Res 10: 436-451.

Dev VG, Miller DA, Miller OJ. 1973. Chromosome markers in Mus musculus strain differences in C-banding. Genetics 75: 663-670.

Eicher EM. 1971. The identification of the chromosome bearing linkage group XI1 in the mouse. Genetics 69: 267-271.

Flamm WG. 1972. Highly repetitious sequences of DNA in chromosomes. Int Rev Cytol 32:1-51.

Forejt F. 1973. Centromeric heterochromatin polymorphism in the house mouse. Chromosoma 43: 187-201.

Jones K. 1970. Chromosomal and nuclear location of mouse satellite DNA in individual cells. Nature 225: 912-915.

Joseph A, Mitchell AR, Miller OJ. 1989. The organisation of the mouse satellite DNA at centromeres. Exp Cell Res 183: 494-500.

Kit S. 1961. Equilibrium sedimentation in density gradients of DNA preparations from animal tissues. J  Mol Biol 3: 711-716.

Malcon A, Smith F, Trifonor V. 2007. Mammalian karyotype evolution. Nature 63: 950-961.

Pardue ML, Gall JG. 1969. Molecular hybridisation of radioactive DNA to the DNA of cytological preparations. Proc Nat Acad Sci USA 64: 600-604.

Pietras DF, Bennett KL, Siracusa LD, Woodworth-Gutai M, Chapman VM, Gross KW, Kane-Haas C, Hastie ND. 1983. Construction of a small Mus musculus repetitive DNA library: identification of a new satellite sequence in Mus musculus. Nucleic Acids Res 11: 6965-6983.

Guenet JL, Bonhomme F. 2003. Wild mice an ever increasing contribution to a popular mammalian model. Trends Genet 19: 5467-5472.

Gunduz I. 2010. Staggered chromosomal hybrid zones in the house mouse: Relevance to reticulate evolution and speciation. Genes 1: 193-209.

Rajabi-mahan H, Orth A, Siahsarvie R, Boursot P, Darvish J, Bonhomme F. 2012. The south-eastern house mouse Mus musculus castaneus (Rodentia:Muridea) is a polytypic subspecies. Biol J Linn Soc Lond 107: 295-306.

Romanenko SA, Volobouev VT, Perelman PL, Lebedev VS, Serdukova NA, Trifonov VA, Biltueva LS, Nie V, Obrien PCM, Bulatova NS, Ferguson-Smith MA, Yang F, Graphodatsky AS. 2007. Karyotype evolution and phylogenetic relationships of hamsters (Cricetidae, Muroidea, Rodentia) inferred from chromosomal painting and banding comparison. Chromosome Res 15: 283-297.

Sharma T, Bardhan A, Bardhan M. 2003. Reduced meiotic fitness in hybrids with heterozygosity for heterochromatin in the speciating Mus Trricolor complex. Bioscience 28: 137-143.

Southerne M. 1970. Base sequence and evolution of guinea pig a-satellite DNA. Nature 227: 794-798.

Vig BK, Richards BT. 1992. Formation of primary constriction and heterochromatin in mouse does not require minor satellite DNA. Exp Cell Res 201: 292-298.

Wong AK, Rattner JB. 1988. Sequence organisation and cytological localization of the minor satellite of mouse. Nucleic Acids Res 16: 11645-11661.