A Modified Method to Assess Secondary Dormancy in the Seeds of Different Rapeseed Lines and Cultivars

Document Type : Research Article

Authors

1 Seed and Plant Certification and Registration Institute, Agricultural Research, Education and Extension Organization, Karaj, Alborz, Iran

2 Department of Agronomy, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran

3 Research Institute of Agriculture and Natural Resources of Golestan Province, Gorgan, Iran

4 Department of Biology, Golestan University, Gorgan, Iran

Abstract

The exposure of seeds to undesirable soil conditions induces secondary dormancy and this causes many problems for seed producers. Assessing seed secondary dormancy potential is an important factor in developing rapeseed lines and cultivars. A relatively rapid, precise, and reproducible method derived from the Hohenheim standard dormancy test (HSDT) has been developed for secondary dormancy evaluation of 41 rapeseeds (Brassica napus L.) lines and 5 cultivars. The two previous methods, HSDT and rapid secondary dormancy (RDT) were compared with our suggested fast-reproducible dormancy test (FRDT) method. All three methods consider three stages for seeds i.e. dormancy induction, germination in darkness, and breaking secondary dormancy. In the FRDT method, time to germination in darkness and seed dormancy breakage duration decreased to 7 and 4 days, respectively, whereas the corresponding figures in the HSDT were 14 and 7 days. Meanwhile, the duration of the three stages in sum decreased from 35 to 25 days. The obtained ranges of seed dormancy in different lines and cultivars as assessed by HSDT, FRDT, and RDT varied from 6 to 98.75, 7.5 to 99, and 0 to 36%, respectively. Positive relationships were found between data of the seed secondary dormancy testing by HSDT and FRDT methods. Owe to the precise and reproducible estimates, both HSDT and FRDT methods can be used for testing seed secondary dormancy in different rapeseed lines and cultivars. This method helps seed breeders to improve the screening of new rapeseed lines and cultivars with lower potential for secondary dormancy and as a result reduce the risk of volunteer rapeseed emergence in the field, which compromises yield in the next growing season.

Keywords

References

Benech-Arnold RL, Sanchez RA, Forcella F, Kruk BC, Ghersa CM. 2000. Environmental control of dormancy in weed seed banks in soil. Field Crops Res 67: 105-122.
Benvenuti S, Mazzoncini M. 2021. “Active” weed seed bank: soil texture and seed weight as key factors of burial-depth inhibition. Agronomy 11: 210.
Bonner FT. 1981. Measurement and management of tree seed moisture. New Orleans: USDA Forest Service, Southern Forest Experiment Station.
Buijs. G. 2020. A perspective on secondary seed dormancy in Arabidopsis thaliana. Plants 9: 749. doi: 10.3390/plants9060749
Farshadfar E. 2011. Principles of multivariate statistical methods. Kermanshah: Razi university press. pp. 734. [in Persian].
Fenner M, Thompson K. 2005. The ecology of seeds. Cambridge, UK: Cambridge University Press.
Gruber S, Claupein, W. 2007. Fecundity of volunteer oilseed rape and estimation of potential gene dispersal by a practice-related model. Agric Ecol Environ 119: 401-408.
Gruber S, Pekrun C, Claupein W. 2004. Population dynamics of volunteer oilseed rape (Brassica napus L.) affected by tillage. Eur J Agron 20: 351-361.
Gruber S, Weber EA, Thole H, Mohring J, Dietz-Pfeilstetter A, Claupein W. 2018. Impact of cultivar on survival of volunteer oilseed rape populations in fields is more important than field management. Weed Res 58(2): 89-98.
Gulden RH, Shirtliffe SJ, Thomas AG. 2003. Secondary seed dormancy prolongs persistence of volunteer canola in western Canada. Weed Sci 51: 904-913.
Gulden RH, Thomas AG, Shirtliffe SJ. 2004. Relative contribution of genotype, seed size and environment to secondary seed dormancy potential in Canadian spring oilseed rape (Brassica napus). Weed Sci 44: 97-106.
Haile TA, Shirtliffe SJ. 2014. Effect of harvest timing on dormancy induction in canola seeds. Weed Sci 62: 548-554.
Huang S, Gruber S, Stockmann F, Claupein W. 2016. Dynamics of dormancy during seed development of oilseed rape (Brassica napus L.). Seed Sci Res 26: 1-9.
Kołodziejek J, Patykowski J. 2015. Effect of environmental factors on germination and emergence of invasive Rumex confertus in central europe. Scic World J   170176: 1-10.
Lutman PJW, Berry K, Payne RW, Simpson E, Sweet JB, Champion GT, May MJ, Wightman P, Walker K, Lainsbury M. 2005. Persistence of seeds from crops of conventional and herbicide tolerant oilseed rape (Brassica napus). Proc Royal Soc 272: 1909-1915.
Martel C, Blair LK, Donohue K. 2018. PHYD prevents secondary dormancy establishment of seeds exposed to high temperature and is associated with lower PIL5 accumulation. J Exp Bot 69 (12): 3157-3169.
Michael BE, Kaufman MR. 1973. The osmotic potential of polyethylene glycol 6000. Plant physiol 51: 914-916.
Mollard FPO, Insausti P. 2009. Breaking Setaria parviflora seed dormancy by nitrates and light is part of a mechanism that detects a drawdown period after flooding. Aquat Bot 91: 57-60.
Momoh EJJ, Zhou WJ, Kristiansson B. 2002. Variation in the development of secondary dormancy in oilseed rape genotypes under conditions of stress. Weed Res 42: 446-455.
Pawłowski TA, Bujarska-Borkowska B, Suszka J, Tylkowski T, Chmielarz P, Klupczynska EA, Staszak AM. 2020. Temperature regulation of primary and secondary seed dormancy in Rosa canina L.: Findings from proteomic analysis. Int J Mol Sci 21: 7008. doi: https://doi.org/10.3390/ijms21197008.
Pekrun C, Hewitt JDJ, Lutman PJW, 1998. Cultural control of volunteer oilseed rape (Brassica napus L.). J Agric Sci 130: 155-163.
Pekrun C, Lane PW, Lutman PJW, 1999. Modelling the potential for gene escape in oilseed rape via the soil seedbank: its relevance for genetically modified cultivars. In: Proceedings No. 72 of the BCPC Symposium on Gene flow and Agriculture: Relevance for Transgenic Crops, Staffordshire, UK, 12-14 April 1999, pp. 101-106.
Pekrun C, Lutman PJW, Baeumer K. 1997a. Germination behaviour of dormant oilseed rape seeds in relation to temperature. Weed Res 37: 419-431.
Pekrun C, Lutman PJW, Baeumer K. 1997b. Induction of secondary dormancy in rape seeds (Brassica napus L.) by prolonged imbibition under conditions of water stress or oxygen deficiency in darkness. Eur J Agron 6: 245-255.
Pekrun C. 1994. Untersuchungen zur sekundaren Dormanz bei Raps (Brassica napus L.). Ph.D Thesis, Georg-August University, Gottingen, Germany [in German].
Postma FM, Lundemo S, Agren J. 2016. Seed dormancy cycling and mortality differ between two locally adapted populations of Arabidopsis thaliana. Ann Bot 117: 249-256.
R Core Team. 2014. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria.  URL http://www.R-project.org/.
Schatzki J, Allam M, Kloppel C, Nagel M, Borner A, Mollers C. 2013. Genetic variation for secondary seed dormancy and seed longevity in a set of black-seeded European winter oilseed rape cultivars. Plant Breed 132: 174-179.
Schlink S. 1994. Ökologie der Keimung und Dormanz von Körneraps (Brassica napus L.) und ihre Bedeutung für eine Überdauerung der Samen im Boden. Ph.D. thesis, University of Göttingen, Germany.
Shayanfar A, Ghaderifar F, Behmaram R, Soltani A, Sadeghipour H. 2018. Temperature and light effects on volunteer’s rapeseed (Brassica napus L.) secondary dormancy and germination. J Plant Prot 32(2): 269-278.
Shayanfar A, Ghaderi-Far F, Behmaram R, Soltani A, Sadeghipour HA. 2020. Impacts of fire cues on germination of Brassica napus L. seeds with high and low secondary dormancy. Plant Biol 22: 647-654.
Soltani E, Baskin JM, Baskin CC. 2019. A review of the relationship between primary and secondary dormancy, with reference to the volunteer crop weed oilseed rape (Brassica napus). Weed Res 59: 5-14.
Weber EA, Frick K, Gruber S, Claupein W. 2010. Research and development towards a laboratory method for testing the genotype predisposition of oilseed rape (Brassica napus L.) to secondary dormancy. Seed Sci Technol 38: 298-310.
Weber EA, Gruber S, Claupein W. 2014. Emergence and performance of volunteer oilseed rape (Brassica napus) in different crops. Eur J Agron 60: 33-40.
Weber EA, Gruber S, Stockmann F, Claupein W. 2013. Can low-dormancy oilseed rape (Brassica napus) genotypes be used to minimize volunteer problems? Field Crops Res 147: 32-39.
Zhu YM, Li YD, Colbach N, Ma KP, Wei W, Mi XC. 2012. Seed losses at harvest and seed persistence of oilseed rape (Brassica napus) in different cultural conditions in Chinese farming systems. Weed Res 52: 317-326.
Journal of Genetic Resources
Volume 7, Issue 2
September 2021
Pages 246-255

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