Genetic Relationships of Some Mint Species Using Seed Storage Protein Pattern

Document Type : Research Article

Authors

1 Plant Breeding Department, Agriculture Faculty, Payame Noor University, Tehran, Iran

2 Payame Noor University, Tehran, Iran

Abstract

Mints are herbaceous perennial plants with aromatic leaves that are cultivated for their essential oils. The essential oil of Mint used in pharmaceutical, perfumery and cosmetics applications. The aim of this study was to analyze the relationship between nine different genotypes of Mentha spicata and M. piperita collected from different regions of Iran according to the seed storage protein profile using the SDS-PAGE method. The electropherogram showed a total of 31 bands of proteins with the molecular weight ranging from 12 kDa to 103 kDa. Among the genotypes G1, G5 and G9 showed the maximum number (26) of protein bands while the minimum number (9) of bands was present in genotype G7. Cluster analysis based on the seed storage protein profile, using the Euclidean distance matrix and the UPGMA method, grouped the evaluated genotypes into three clusters. Five genotypes (G1, G4, G5, G8, and G9) were in group 1, group two contained genotype G3 and the rest of the genotypes were in the third group (G2, G6, G7). Cluster analysis showed that some of the genotypes of two different species of M. piperita and M. spicata were closely related to each other and were in the same group. With regard to M. piperita is a natural hybrid of M. spicata and M.aquatica, this result is expected. The protein variability analysis clearly showed that there was divergence among and within the studied genotypes. It was concluded that this study will be useful for the future breeding and conservation program of Mentha genotypes.       

Keywords

References

Ali Z, Qureshi AS, Ali W, Gulzar H, Nisar M, Ghafoor A. 2007. Evaluation of genetic diversity present in pea (Pisum sativum L.) germplasm based on morphological traits, resistance to powdery mildew and molecular characteristics. Pak J Bot 39: 2739-2747.
Alwhibi MS. 2017. Characterization of seed storage protein patterns of Heliotropium digynum. Saudi J Biol Sci 24: 1404-1409.
Areias FM, Valentao P, Andrade PB, Ferreres F, Seabra RM. 2001. Phenolic fingerprint of peppermint leaves. Food Chem 73: 307-311
Badr A, Mustafa AZ, El-galaly MA, Mobarak AA, Hassan MG. 2003. Genetic diversity among Mentha populations in Egypt as reflected by morphological and protein electrophoretic variations. Proceeding 1th conference for Agriculture and Food 1 (1): 269-286.
Bhat S, Maheshwari P, Kumar S, Kumar A. 2002. Mentha species: In vitro regeneration and genetic transformation. Mol Biol Today 3(1): 11-23.
Bompalli LK, Nallabilli L. 2013. Genetic diversity of Ocimum species through biochemical technique and UPGMA cluster analysis. Int J Pharm 5(4): 155-159.
Crawford DJ, Ruiz E, Stuessy TF, Tepe E, Qevequep A, Gonzales F, Jensen RJ, Anderson GJ, Bernardello G, Baeza CM, Swenson U, Silva M. 2001. Allozyme diversity in endemic florewing plant species of the Juan Fernandez Archipelago, Chile: ecological and historical factors with implications for conservation. Am J Boty 88(12): 2195-2203.
El-Ghorab AH. 2006. The chemical composition of Mentha pulegium L. essential oil from Egypt and its antioxidant activity. J Essen Oil Bearing Plants 9: 183-195.
Elham AA, El-Hady A, Atef AA, Haiba Nagwa R, El-Hamid A, Aida A. 2010. Phylogenetic diversity and relationships of some tomato varieties by electrophoretic protein and RAPD analysis. J Am Sci 6 (11): 434-441.
Fenwick AL, Ward SM. 2001. Use of random amplified polymorphic DNA markers for cultivar identification in Mint. Hort Science 36 (4): 761-764.
Gobert V, Moia S, Taberlet P. 2003. Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers. Am J Bot 89: 2017-2023.
Hamrick JL, Godt MJW, Murawski DA, Loveless MD. 1991. Correlations between species traits and allozyme diversity: implication for conservation biology. In: Falk DA. and Holsinger KE (Eds.), Genetics and Conservation of Rare Plants. New York: Oxford University Press. 75-86.
Iqbal SH, Ghafoor A, Ayub N. 2005. Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pak J Bot 37: 87-96.
Jafari, A, Farsi M, Soleimanian Shafa, F, Taghizadeh N. 2009. Seed protein study on some populations of Salvia (Lamiaceae) using electrophoresis technique in North-East of Iran. Asian J Plant Sci 8(4): 322-324.
Javaid A, Ghafoor A, Anwar R. 2004. Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pak J Bot 36: 25-29.
Kakaei M, Kahrizi D. 2011. Study of seed proteins pattern of Brassica napus varieties via sodium dodecyl sulfate polyacrylamide gel electrophoresis. Int Res J Biotechnol 2: 26-28.
Khan N, Ali N, Rabbani MA, and Masood MS. 2010. Diversity of glutelin alpha subunits in rice varieties from Pakistan. Pak J Bot 42: 2051-2057
Khoshsokhan F, Babalar M, Fatahi M, Poormeidani A. 2014. Assessment of genetic diversity of some populations of Thymus kotschyanus using RAPD molecular markers. Cercetari Agronomice in Moldova 3 (159): 71-81.
Kumar OK, Tata SS. 2010. SDS-PAGE seed storage protein profiles in Chili pappers. Not Sci Biol 2 (3): 86-90.
Lawrence BM. 2007. Mint: the genus Mentha., New York: Tylor and Francis group, Bocan Raton.
Masoumi SM, Kahrizi D, Rostami-Ahmadvandi H, Soorni J, Kiani S, Mostafaie A, Yari K. 2012. Genetic diversity study of some medicinal plant accessions belongs to Apiaceae family based on seed storage proteins patterns. Mol Biol Rep 39 (12): 10361-10365.
Momeni S, Shiran B, Razmjoo K. 2006. Genetic variation in Iranian Mints on the bases of RAPD analysis. Pak Biol Sci 9 (10): 1898-1904.
Murray MJ, Lincol DE, Marble PM. 1972. Oil composition of Mentha aquatic × Mentha spicata F1 hybrids in relation to the origin of Mentha piperita. Can J Genet Cytol 14: 13-29.
Nisar M, Ghafoor A, Khan MR, Siddiqui SU, Khan N, Siddiqui MF. 2011. Novel protocol for albumin and globulin detection in Pisum sativum genotypes using sodium plant proteome. Pak J Bot 43 (3): 1733-1734.
Rabbani MA, Quershi AS, Azfal M, Anwar R and Komatsu. 2001. Characterization of mustard (Brassica juncea (L.) Czern. & Coss.) germplasm by SDS-PAGE of total seed proteins. Pak J Bot 33(2): 173-179.
Rahman MM, Hirata Y. 2004. Genetic diversity in Brassica species using SDS-PAGE analysis. J Biol Sci 4: 234-238.
Safaei M, Sheidai M, Alijanpoor B, Noormohammadi Z. 2016. Species delimitation and genetic diversity analysis in Salvia with the use of ISSR molecular markers. Acta Bot Croat 75 (1): 45-52.
Šarić-Kundalić B, Fialová S, Dobeš C, Ölzant S, Tekeľová D, Grančai D, Reznicek G, Saukel J. 2009. Multivariate numerical taxonomy of Mentha Species, hybrids, varieties and cultivars. Sci Pharm 77: 851-876.
Shah SMA, Rahman H, Abbasi FM, Rabbani MA, Khan IA, Shinwari ZK. 2011. Inter specific variation of total seed protein in wild rice germplasm using SDS-PAGE. Pak J Bot 43(4): 2147-2152.
Shasany AK, Darokar MP, Dhawan S, Gupta AK, Gupta S, Shukla AK, Patra NK. 2005. Use of RAPD and AFLP markers to identify inter and intraspecific hybrids of Mentha. J Hered 31: 1-8.
Shinwari S, Akbar F, Rabbani MA, Mumtaz AS, Shinwari ZK. 2013. Evaluation of genetic diversity in different genotypes of Eruca sativa from Pakistan by SDS-PAGE analysis. Pak J Bot 45: 1235-1240.
Shinwari ZK, Sultan S and Mahmood T. 2011. Molecular and morphological characterization of selected Mentha spicata. Pak J Bot 43 (3): 1433-1436.
Stoyanova M, Ivanov P, Penchev E. 1992. Electrophoretic Criteria for Protein in Seeds of Bean Cultivars. Genet Breed 25: 28-36.
Taghiloofar AH, Anghourani HR, Nasi HN. 2014. Evaluation of genetic diversity among some Iranian spearmint (Mentha spicata L) accessions using morphological traits. Int J Agri Crop Sci 7 (11): 898-907.
Vishwanath K, Prasanna KPR, Pallvi HM, Prasad S, Ramegowda DPJ. 2011. Identification of tomato (Lycopersicon esculentum) varieties through total soluble seed proteins. Res J Agr Sci 2(1): 8-12.
Xi J, Wang X, Li S, Zhou X, Yue L, Fan J, Hao D. 2006. Polyethylene glycol fractionation improved detection of low abundant proteins by two-dimensional electrophoresis analysis of plant proteome. Phytochemistry 67: 2341-2348
Yousef RMM, El-Leel FA, Mohamed SY. 2015. Comparative study for some Mentha species and varieties under sandy soil conditions. J Appl Sci 15 (2): 355-366.
Zinodini A, Farshadfar M, Safari H, Moradi F, Shirvani H. 2013. Study of genetic relationships of some Mint species using ISSR markers. J Crop Biotechnol 3 (5): 11-21.
 
 
Journal of Genetic Resources
Volume 6, Issue 1
February 2020
Pages 12-19

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