Effect of Different Fatty Acids Sources on Some Blood Factors and Interleukin Gene Expression in Finishing Lambs

Document Type : Research Article

Authors

1 Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Animal Science, Karaj Branch, Islamic Azad University, Karaj, Iran

Abstract

The current research was conducted to study the effects of different fatty acids sources in the diet on blood metabolites and hormones in finishing lambs experimentally infected with the virus that causes foot and mouth disease. A total of fifteen Sangsari male lambs with an average live weight of 48±2 kg and an average age of 8±1 month were randomly assigned to one of three dietary experimental treatments as follows: 1) Calcium soap of palm oil fatty acids (PO) as a source of palmitic acid (16:0); 2) Calcium soap of sunflower oil fatty acids (SO) as the source of linoleic acid (n-6 18:2); and 3) Calcium soap of linseed oil fatty acids (LO) as the source of α-linolenic acid (n-3 18:3). The lambs were housed in individual pens and offered the iso-caloric and iso-nitrogenous diets for 28 days including 21 days of adaptation period and 7 days of the sampling period. The results illustrated that the lowest and the highest expression of IL-4 mRNA were measured in LO and SO treatments, respectively. Expression of IL-8 mRNA was lower in LO and PO treatments when compared with SO. The highest level of glucose in LO treatment when compared with sunflower oil or palm oil. Lambs on the LO diet showed the highest blood concentration of insulin and the lowest blood concentration of glucagon when compared with lams on SO and PO diets. The highest blood contents of triiodothyronine and thyroxin hormones were measured in lambs on the LO diet when compared with other treatments. However, the concentration of blood glucose, insulin, glucagon, triiodothyronine, and thyroxin were the same between PO and SO groups. In conclusion, the findings of the current experiment confirmed that the inclusion of α-linolenic acid but not linoleic acid in the diet of virus-infected lambs suppressed pro-inflammation with lowering expression of IL-4 and IL-8 mRNA and increased blood glucose, insulin, T3, and T4 which may lead to higher weight gain and feed efficiency of virally infected lambs.

Keywords

References

Al-Hasani H, Joost HG. 2005. Nutrition-/diet-induced changes in gene expression in white adipose tissue. Best Pract Res Clin Endocrinol Metabol 19: 589-603.
Araújo JP, Pires P, Cerqueira JL, Barros M, Moreno T. 2020. Intramuscular fatty acid composition of the longissimus muscle of unweaned minhota breed calves at different slaughter age. Iran J Appl Anim Sci 10 (1): 17-24.
Berge RK, Madsen L, Vaagenes H, Tronstad KJ, Gottlicher M, Rustan AC. 1999. In contrast with docosahexaenoic acid, eicosapentaenoic acid and hypolipidaemic derivatives decrease hepatic synthesis and secretion of triacylglycerol by decreased diacylglycerol acyltransferase activity and stimulation of fatty acid oxidation. Biochem J 343: 191-197.
Bertoni G, Minuti A, Trevisi E. 2015. Immune system, inflammation and nutrition in dairy cattle. Anim Prod Sci 55: 943-948.
Bhaswant M, Poudyal H, Brown L. 2015. Mechanisms of enhanced insulin secretion and sensitivity with n-3 unsaturated fatty acids. J Nutr Biochem 26: 571-584.
Bhathena SJ. 2000. Relationship between fatty acids and the endocrine system. Biofactors 13, 35-39.
Bhathena SJ. 2006. Relationship between fatty acids and the endocrine and neuroendocrine system. Nutr Neurosci 9: 1-10.
Boelen A, Platvoet-ter Schiphorst M, Wiersinga W. 1996. Relationship between serum 3, 5, 3′-triiodothyronine and serum interleukin-8, interleukin-10 or interferonγ in patients with nonthyroidal illness. J Endocrnol Investig 19: 480-483.
Calder PC. 2003. N- 3 polyunsaturated fatty acids and inflammation: from molecular biology to the clinic. Lipids 38: 343-352.
Calder PC, Yaqoob P, Thies F, Wallace FA, Miles E.A. 2002. Fatty acids and lymphocyte functions. Br J Nutr 87: 31-48.
Capen CC, Martin SL. 1989. The effects of xenobiotics on the structure and function of thyroid follicular and c-cells. Toxicol Pathol 17: 266-293.
Ceciliani F, Ceron J, Eckersall P, Sauerwein H. 2012. Acute phase proteins in ruminants. J Proteomics 75: 4207-4231.
Cooper SL, Sinclair LA, Wilkinson RG, Hallett KG, Enser M, Wood JD. 2004. Manipulation of the n-3 polyunsaturated fatty acid content of muscle and adipose tissue in lambs. J Anim Sci 82 (5): 1461-1470.
Darwesh AM, Sosnowski DK, Lee TY, Keshavarz-Bahaghighat H, Seubert JM. 2019. Insights into the cardioprotective properties of n-3 pufas against ischemic heart disease via modulation of the innate immune system. Chem Biol Interact 308: 20-44.
Ebrahimi M, Rajion MA, Goh YM. 2014. Effects of oils rich in linoleic and α-linolenic acids on fatty acid profile and gene expression in goat meat. Nutrients 6: 3913-3928.
Faisal SM, Chen JW, Yan F, Chen TT, Useh NM, Yan W, Guo S, Wang SJ, Glaser AL, McDonough SP. 2013. Evaluation of a mycobacterium avium subsp. Paratuberculosis leud mutant as a vaccine candidate against challenge in a caprine model. Clin Vaccine Immunol 20: 572-581.
Fisher D, Chopra I, Dussault J. 1972. Extrathyroidal conversion of thyroxine to triiodothyronine in sheep. Endocrinol 91: 1141-1144.
Ghaderzadeh S, Mirzaei Aghjegheshlagh F, Nikbin S, Navidshad B. 2019. Correlation effects of nano selenium and conjugated linoleic acid on the performance, lipid metabolism and immune system of male Moghani lambs. Iran J Appl Anim Sci 9 (3): 443-451.
Gulliver CE, Friend MA, King BJ, Clayton E.H. 2012. The role of omega-3 polyunsaturated fatty acids in reproduction of sheep and cattle. Anim Reprod Sci 131: 9-22.
Hadfield JM. 2017. Characterization of the immune response to lipopolysaccharide in early pregnant ewes as a model to study bacterial infection induced embryonic loss. Graduate Theses, Dissertations, and Problem Reports. 5731.
Halakoo G, Teimouri Yansari A, Mohajer M, Chashnidel Y. 2020. Effect of different fat sources on some blood metabolites, hormones, and enzyme activities of lambs with different residual feed intake in heat-stressed condition. Iran J Appl Anim Sci 10 (4): 657-667.
Huszenicza G, Kulcsar M, Rudas P. 2002. Clinical endocrinology of thyroid gland function in ruminants. Vet Med 47: 199-210.
Ikeda I, Cha JY, Yanagita T, Nakatani N, Oogami K, Imaizumi K, Yazawa, K. 1998. Effects of dietary α-linolenic, eicosapentaenoic and docosahexaenoic acids on hepatic lipogenesis and β-oxidation in rats. Biosci Biotechnol Biochem 62: 675-680.
Itoh Y, Kawamata Y, Harada M, Kobayashi M, Fujii R, Fukusumi S, Ogi K, Hosoya M, Tanaka Y, Uejima H. 2003. Free fatty acids regulate insulin secretion from pancreatic β cells through gpr40. Nature 422: 173-176.
Jamshidi K, Zahedi A, Poorghasemi M, Seidavi AR. 2020. Slaughterhouse study on the prevalence and pathological lesions caused by Dictyocaulus viviparus infection in cattle and water buffaloes. Iran J Med Microbiol 14 (6): 584-595.
Kaveh M, Eftekhar N, Boskabady MH. 2019. The effect of alpha linolenic acid on tracheal responsiveness, lung inflammation, and immune markers in sensitized rats. Iranian J Basic Medical Sci 22: 255-261.
Kong WM, Martin NM, Smith KL, Gardiner JV, Connoley IP, Stephens DA, Dhillo WS, Ghatei MA, Small CJ, Bloom SR. 2004. Triiodothyronine stimulates food intake via the hypothalamic ventromedial nucleus independent of changes in energy expenditure. Endocrinol 145: 5252-5258.
Kumamoto T, Ide T. 1998. Comparative effects of α‐and γ‐linolenic acids on rat liver fatty acid oxidation. Lipids. 33: 647-654.
Kurotani K, Sato M, Ejima Y, Nanri A, Yi S, Pham NM, Akter S, Poudel-Tandukar K, Kimura Y, Imaizumi K. 2012. High levels of stearic acid, palmitoleic acid, and dihomo-γ-linolenic acid and low levels of linoleic acid in serum cholesterol ester are associated with high insulin resistance. Nutr Res 32: 669-675.
Ladeira MM, Schoonmaker JP, Gionbelli MP, Dias JC, Gionbelli TR, Carvalho JRR, Teixeira PD. 2016. Nutrigenomics and beef quality: a review about lipogenesis. Int J Mol Sci 17: 918. https://doi.org/10.3390/ijms17060918.
Lakdawala N, Grant-Kels JM. 2015. Acrodermatitis enteropathica and other nutritional diseases of the folds (intertriginous areas). Clin Dermatol 33: 414-419.
Loor JJ, Bertoni G, Hosseini A, Roche JR, Trevisi E. 2013. Functional welfare–using biochemical and molecular technologies to understand better the welfare state of peripartal dairy cattle. Anim Prod Sci 53: 931-953.
Madsen L, Rustan AC, Vaagenes H, Berge K, Dyrøy E, Berge, RK. 1999. Eicosapentaenoic and docosahexaenoic acid affect mitochondrial and peroxisomal fatty acid oxidation in relation to substrate preference. Lipids 34: 951-963.
Matsuyama W, Mitsuyama H, Watanabe M, Oonakahara KI, Higashimoto I, Osame M, Arimura K. 2005. Effects of omega-3 polyunsaturated fatty acids on inflammatory markers in copd. Chest 128: 3817-3827.
Mizota T, Fujita‐Kambara C, Matsuya N, Hamasaki S, Fukudome T, Goto H, Nakane S, Kondo T, Matsuo H. 2009. Effect of dietary fatty acid composition on th1/th2 polarization in lymphocytes. J Parent Enter Nutr 33: 390-396.
Muramatsu T, Yatsuya H, Toyoshima H, Sasaki S, Li Y, Otsuka R, Wada K, Hotta Y, Mitsuhashi H, Matsushita K. 2010. Higher dietary intake of alpha-linolenic acid is associated with lower insulin resistance in middle-aged japanese. Prev Med 50: 272-276.
Orsel K, Dekker A, Bouma A, Stegeman JA, De Jong MCM. 2007. Quantification of foot and mouth disease virus excretion and transmission within groups of lambs with and without vaccination. Vaccine 25: 2673-2679.
Park JH, Kim SJ, Oem JK, Lee KN, Kim YJ, Kye SJ, Park JY, Joo YS. 2006. Enhanced immune response with foot and mouth disease virus vp1 and interleukin-1 fusion genes. J Vet Sci 7: 257-262.
Poorghasemi M, Chamani M, Mirhosseini SZ, Sadeghi AA, Seidavi A. 2017a. Effect of probiotic and different sources of fat on performance, carcass characteristics, intestinal morphology and ghrelin gene expression on broiler chickens. Kafkas Univ Vet Fak Derg 24 (2): 169-178.
Poorghasemi M, Chamani M, Mirhosseini SZ, Sadeghi AA, Seidavi A. 2017b. Effect of lactofeed probiotic and different sources of fat on performance, carcass characteristics and lipid parameters in broiler chickens. J Livest Sci 8: 1-7.
Poorghasemi M, Seidavi AR, Qotbi AAA, Chambers JR, Laudadio V, Tufarelli V. 2015. Effect of dietary fat source on humoral immunity response of broiler chickens. European Poult Sci 79: 1-8.
Poorghasemi M, Seidavi AR, Qotbi AAA. 2013a. Investigation on fat source effects on broiler chickens performance. Res J Biotechnol 8 (1): 78-82.
Poorghasemi M, Seidavi AR, Qotbi AAA, Laudadio V, Tufarelli V. 2013b. Influence of dietary fat source on growth performance responses and carcass traits of broiler chicks. Asian-Australas J Anim Sci 26 (5): 705-710.
Powanda M. 1980. Host metabolic alterations during inflammatory stress as related to nutritional status. Am J Vet Res 41: 1905-1911.
Rajaei-Sharifabadi H, Naserian AA, Nassiry MR, Bottje W. 2021. Relationship of mitochondrial biogenesis and phenotypic expression of residual feed intake in fat-tailed lambs. Iran J Appl Anim Sci 11 (1): 117-122.
Rozing MP, Westendorp RG, Maier AB, Wijsman CA, Frölich M, De Craen AJ, Van Heemst D. 2012. Serum triiodothyronine levels and inflammatory cytokine production capacity. Age 34: 195-201.
Sandri E, Camera M, Sandri E, Harvatine KJ, De Oliveira D. 2018. Peroxisome proliferator-activated receptor gamma (pparγ) agonist fails to overcome trans-10, cis-12 conjugated linoleic acid (cla) inhibition of milk fat in dairy sheep. Anim 12: 1405-1412.
Slozhenkina MI, Gorlov IF, Shakhbazova OP, Radjabov RG, Ivanova NV, Mosolova DA, Knyazhechenko OA, Poorghasemi M, Seidavi  A. 2020. Productivity of steers of different genotypes: forecast based on interior indicators. Arq Bras Med Vet Zootec 72 (6): 2279-2287.
Souza LL, Nunes MO, Paula GS, Cordeiro A, Penha-Pinto V, Neto JFN, Oliveira KJ, do Carmo MdGT, Pazos-Moura CC. 2010. Effects of dietary fish oil on thyroid hormone signaling in the liver. J Nutr Biochem 21: 935-940.
Trevisi E, Amadori M, Bakudila A, Bertoni G. 2009. Metabolic changes in dairy cows induced by oral, low-dose interferon-alpha treatment. J Anim Sci 87: 3020-3029.
Wei D, Li J, Shen M, Jia W, Chen N, Chen T, Su D, Tian H, Zheng S, Dai Y. 2010. Cellular production of n-3 pufas and reduction of n-6 to n-3 ratios in the pancreatic β-cells and islets enhance insulin secretion and confer protection against cytokine-induced cell death. Diabetes. 59: 471-478.
Williamson JR, Kreisberg R, Felts P. 1966. Mechanism for the stimulation of gluconeogenesis by fatty acids in perfused rat liver. Proc Natl Acad Sci USA 56: 247-254.
Winnik S, Lohmann C, Richter EK, Schäfer N, Song WL, Leiber F, Mocharla P, Hofmann J, Klingenberg R, Borén J. 2011. Dietary α-linolenic acid diminishes experimental atherogenesis and restricts t cell-driven inflammation. Eur Heart J 32: 2573-2584.
Yaqoob P, Pala H, Cortina-Borja M, Newsholme EA, Calder PC. 2000. Encapsulated fish oil enriched in alpha-tocopherol alters plasma phospholipid and mononuclear cell fatty acid compositions but not mononuclear cell functions. Eur J Clin Investig 30: 260-274.
Zeng YY, Jiang WD, Liu Y, Wu P, Zhao J, Jiang J, Kuang SY, Tang L, Tang WN, Zhang YA. 2016. Dietary alpha-linolenic acid/linoleic acid ratios modulate intestinal immunity, tight junctions, anti-oxidant status and mRNA levels of nf-κb p65, mlck and nrf2 in juvenile grass carp (ctenopharyngodon idella). Fish Shellfish Immunol 51: 351-364.
Zhao FQ, Moseley WM, Tucker HA, Kennelly JJ. 1996. Regulation of glucose transporter gene expression in mammary gland, muscle, and fat of lactating cows by administration of bovine growth hormone and bovine growth hormone-releasing factor. J Anim Sci 74: 183-189.
Journal of Genetic Resources
Volume 7, Issue 2
2021
Pages 256-264

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