Morpho-physiological Characteristics of Brassica napus L. Under Cadmium Stress

Document Type : Research Article

Authors

Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

Abstract

Cadmium as a heavy metal is also considered as a serious threat for the environment. This element is accumulated in soil through industrial processes as well as phosphate fertilizers and aggregates into the food chain. This study was conducted in order to investigate the different levels of cadmium effects on growth features of rapeseed (Brassica napus L.) plants. Root and shoot weight and height, contents of chlorophyll a and b, total chlorophyll, carotenoid and peroxidase enzyme activity were measured after one month of planting. Generally, an increase in cadmium concentration showed a significant effect on morphological characteristics of root and shoot but revealed no significant effect on chlorophyll a and b as well as on carotenoid contents, though it caused a significant decline in peroxidase activity level specially for concentrations above 90 µM of cadmium treatment. 

Keywords


Arnon DL, 1956. Photosynthesis by isolated chloroplasts IV. Genral concept and comparsion of three photochemical reactions. Biocbem Biopbys Acta 20: 499-461.
Chen X, Wang J, Shi Y, Zhao MQ, Chi GY. 2011. Effects of cadmium on growth and photosynthetic activities in pakchoi and mustard. Bot Stud 52: 41-46.
Cho UH, Park JO. 2000. Mercury-induced oxidative stress in tomato seedlings. Plant Sci 156: 1-9.
Chugh L, Sowhney S. 1996. Effect of cadmium on germination, amylases and rate of respiration of germinating pea seeds. Environ Pollut 92: 1-5.
Daud MK, Sun Y, Dawood M, Hayat Y, Variath MT, Wu YX, Raziuddin, Mishkat U, Salahuddin, Najeeb U, Zhu S. 2009. Cadmium-induced functional and ultrastructural alterations in roots of two transgenic cotton cultivars. J Hazard Mater 161: 463- 473.
Ernst W. 2002. Biochemical aspects of cadmium in plants. In: J. Nriagu ed.  Cadmium in the Environment. Ed., Wiley and Sons, New York, 639-653.
Ewaise EA. 1997. Effects of cadmium, nickel and lead on growth, chlorophyll content and proteins of weed. Biol Plantarum 39: 403-410.
Fusconi A, Gallo C, Camusso W. 2007. Effect of cadmium on root apical meristems of Pisumsativum L.: cell viability, cell proliferation and microtubule pattern as suitable makers for assessment of stress pollution. Mutat Res Genet Toxicol Environ Mutagen 632: 9–19.
Ghahraman A. 1993. Chromophytes of Iran. Volume 2, Tehran University Press.
Ghani A. 2010. Effect of cadmium toxicity on the growth and yield components of mungbean (Vigna radiate (L.) Wilczek). World Appl Sci J 8: 26-29.
Golia EE, Dimirkou A, Mitsios IK. 2008. Influence of some soli parameters on heavy metals accumulation by vegetables grown in Agricultural Soils of Different Soil Orders. B Environ Contam Tox 81: 80-84
Gouia H, Ghorbal MH, Meyer C. 2001. Effect of cadmium on activity of nitrate reductase and on other enzymes of the nitrate assimilation pathway in bean. Plant Physiol 38: 629-638.
Greger M, Ogren E. 1991. Direct and indirect effects of Cd2+ on photosynthesis in sugar beet (Beta vulgaris). Physiol Plantarum 83: 129–135.
Hegedus A, Erdi S, Horvath G. 2001. Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedling under cadmium stress. Plant Sci 160: 1085- 1093.
Jason A. 2003. Chlorophyll and Cartenoid: Handbook of Physiological Method. Cambridge University Press, Cambridge, New York.
Jeliazkova EA, Craker LE, Xing B. 2003. Seed germination of anise, caraway, and fennel in heavy metal contaminated solutions. J Herbs Spices Med Plants 10: 83-93.
Karimi GH, Nojavan M. 2007. Effects of cadmium chloride on growth parameters of lense miller seedlings. J Agr Husbandry res 76: 46-52.
Lee K, Gunningham B, Paulsen G, Liang G, Moore R. 2005. Effects of cadmium on respiration rate and activities of several enzymes in soybean seedlings. Physiol Plantarum 36: 4–6.
MacAdam JW, Nelson sharp CJ. 1992. Peroxidase activity in the leaf elongation zone of tall fescue. Plant Physiol 99: 872- 878.
Maleki A, Zarasvand MA. 2008. Heavy metals in selected edible vegetables and estimation of their daily intake in Sanandaj, Iran. South East Asian J Trop Med Public Health 39: 335-340.
Mejáre M, Bülow L. 2001. Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals. Trends Biotechnol 19: 67-73.
Minooi S, MinaiTehrani D, Samiee K, Farivar SH. 2008. Effects of cadmium on macroscopic and microscopic variations in Chlorophytum comosum. Iran J Biol 21: 737-747.
Okuda T, Matsuda Y, Yamanaka A, Sagisaka S. 2004. Abrupt increase in the level of hydrogen peroxide in leaves of winter wheat in caused by cold treatment. Plant Physiol 97: 1265–1267.
Omale J, Emmanuel U C. 2011. Comparative studies on the protein and mineral composition of some selected Nigerian vegetables. Afr J Food Sci 5: 22-25
Ostad Sharif M, Keyhani A, Shahsavan Behboudi B, MinaiTehrani D. 2001. Effects of Cd on morphological characteristics of saffron root. Tehran U J Sci 27: 207-225
Sanita di Toppi L, Gabbrielli R. 1999. Response to cadmium in higher plants- review. Environ Exp Bot 41: 105-130.
Shanker A, Cervantes KC, Tavera HL. 2005. Cromium toxicity in plants. Environ Int 31: 739- 753.
Shahidi.A, Sepehr K. 2002. Canola's louses. Publ Agr ministry 19: 59-70.
Shariat A, Osare MH, Ghamari Zare A. 2010. Effect of Cd on physiologic characteristics of Eucalyptus occidentalis, J Agr Nat Resour Water Soil Sci 53: 145-153.
Sing Anil K. 2006. Phytotoxicity of cadmium in paddy (Oryza sativa L.) plants. J Environ Biol 27: 283-285.
Soltani F, Ghorbanli M, Manochehri Kalantari KH. 2006. Effects of Cd on Brassica napus L. photosynthetic pigments, hydrocarbons and malondialdehyde. Iran J Biol 19: 136-145.
Stoyanova D, Chakalova E. 2001. The effect of cadmium on the structure of photosynthetic apparatus in Elodea Canadensis Rich. Plant Physiol 16: 18-26.
Tokalioglu S, Kartal S. 2006. Statistical evaluation of the bioavailability of heavy metals from contaminated soils to vegetables. B Environ Contam Tox 76: 311-9.
Van Assche F, Clijsters H.1990. Effects of metals on enzyme activity in plants. Plant Cell Environ 13: 195-206.  
Vassilev A., Yordanov, I. 1997. Reductive analysis of factors limiting growth of cadmium–treated plants. Bulg J Plant Physiol 23: 114–133.
Zhang F, Shi W, Jim Z, Shen Z. 2003. Response of antioxidative enzymes in cucumber chloroplasts to cadmium toxicity. J Plant Nutr 26: 1779-1788.