Selecting High Zinc-efficient and Assessment of Zinc Stress Tolerance of the Wheat Durum Genotypes

Document Type : Research Article


1 Ph.D. of Agronomy (Crop Physiology), Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, P.O. Box 55181-83111, Maragheh, Iran.

2 Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, P.O. Box 55181-83111, Maragheh, Iran.



At percent, Zn stress tolerance using novel genetic resources is an important mitigation strategy for plant breeding. In this study, thirty-five durum wheat genotypes with different growth habits were evaluated under normal (non-stress) and Zn deficient stress during the 2014-15 cropping season. A total of ten Zn stress tolerance indices including stress tolerance index (STI), relative zinc-deficient index (RDI), yield index (YI), yield stability index (YSI), zinc-deficient resistance index (DI), abiotic tolerance index (ATI), stress susceptibility percentage index (SSPI), sensitive zinc-deficient index (SDI), and modified stress tolerance index (MSTI; K1STI and K2STI) were estimated. Results showed the significant influences of Zn stress on grain yield, as well as significant differences among genotypes for grain yield and the indices. The genotype G33 produced the highest grain yield under normal conditions by 0.854 g plant-1 while genotype G32 had the highest yield by 0.686 g plant-1 under Zn stress conditions. The genotypes G6, G13, G23, and G32 had less grain yield fluctuation, and G1, G21, and G29 genotypes had high grain yield fluctuation in two conditions. Cluster analysis showed that the genotypes, based on indices tended to four groups: tolerant, semi-tolerant, semi-sensitive, and sensitive genotypes, including 10, 17, 7, and 1 wheat genotypes, respectively. Grain yield was strongly positively correlated with STI, YI, DI, K1STI, and K2STI under two conditions, while negatively correlated grain yield with SSPI and SDI in Zn deficit stress condition, respectively. Using STI, YI, DI, K1STI, and K2STI, the genotypes G32, G33, and G19 were found to be the best genotypes with relatively high yield and suitable for both normal and Zn deficits stressed conditions. Therefore, they may be recommended to cultivate in Zn deficit prone regions of the world and also can be used in wheat breeding programs aimed at improving Zn stress tolerance.


Abdoli M, Esfandiari E, Aliloo AA, Sadeghzadeh B, Mousavi SB. 2019. Study of genetic diversity in different wheat species with various genomes based on morphological characteristics and zinc use efficiency under two zinc-deficient growing conditions. Acta Agric Slov 113: 147-161.
Abdoli M, Esfandiari E, Sadeghzadeh B, Mousavi SB. 2016. Zinc application methods affect agronomy traits and grain micronutrients in bread and durum wheat under zinc-deficient calcareous soil. YYU J Agr Sci 26: 202-214.
Abdoli M, Esfandiari E. 2017. Assessment of genetic variation and zinc deficient tolerance in spring durum wheat (Triticum durum Desf.) genotypes in calcareous soil with zinc deficiency. J Genet Resour 3: 7-17.
Agili S, Nyende B, Ngamau K, Masinde P. 2012. Selection, yield evaluation, drought tolerance indices of orange-flesh sweet potato (Ipomoea batatas Lam) hybrid clone. J Nutr Food Sci 2: 1-8.
Akcura M, Ceri S. 2011. Evaluation of drought tolerance indices for selection of Turkish oat (Avena sativa L.) landraces under various environmental conditions. Zemdirbyste 98: 157-166.
Alloway BJ. 2008. Zinc in Soils and Crop Nutrition. Brussels, Belgium: International Zinc Association.
Amiri R, Bahraminejad S, Sasani Sh, Ghobadi M. 2014. Genetic evaluation of 80 irrigated bread wheat genotypes for drought tolerance indices. Bulg J Agric Sci 20: 101-111.
Anwaar HA, Perveen R, Mansha MZ, Abid M, Sarwar ZM, Aatif HM, Umar U, Sajid M, Aslam HMU, Alam MM, Rizwan M, Ikram RM, Alghanem SMS, Rashid A, Khan KA. 2020. Assessment of grain yield indices in response to drought stress in wheat (Triticum aestivum L.). Saudi J Biol Sci 27: 1818-1823.
Arisandy P, Bayuardi Suwarno W, Azrai M. 2017. Evaluation of drought tolerance in maize hybrids using stress tolerance indices. Int J Agron Agric Res 11: 46-54.
Bagci SA, Ekiz H, Yilmaz A, Cakmak I. 2007. Effects of zinc deficiency and drought on grain yield of field-grown wheat cultivars in Central Anatolia. J Agron Crop Sci 193: 198-206.
Bellague D, M’Hammedi-Bouzina M, Abdelguerfi A. 2016. Measuring the performance of perennial alfalfa with drought tolerance indices. Chil J Agric Res 76: 273-284.
Bouslama M, Schapaugh WT. 1984. Stress tolerance in soybean. I. Evaluation of three screening techniques for heat and drought tolerance. Crop Sci 24: 933-937.
Ekbic E, Cagiran C, Korkmaz K, Arsal Kose M, Aras V. 2017. Assessment of watermelon accessions for salt tolerance using stress tolerance indices. Cienc Agrotec 41: 616-625.
Esfandiari E, Abdoli M, Sadeghzadeh B, Mousavi SB. 2018a. Evaluation of Turkish durum wheat (Triticum turgidum var. durum) genotypes based on quantitative traits and shoot zinc accumulation under zinc-deficient calcareous soil. Iranian J Plant Physiol 8: 2525-2537.
Esfandiari E, Abdoli M, Sadeghzadeh B. 2018b. Evaluation of genetic diversity of durum wheat genotypes (Triticum turgidum var durum) using agro-morphological traits for resistance to zinc deficient stress. Res Crop Ecophysiol 13: 23-40.
Esfandiari E, Abdoli M. 2017. Variations of grain yield and agro-morphological traits of some promising durum wheat lines (Triticum turgidum L. var. durum) at zinc sufficient and deficient conditions. J Genet Resour 3: 68-79.
FAO. 2014. Food Supply Database 2014 of Food and Agriculture Organization. Available at: on October 15, 2014.
Farshadfar E, Elyasi P. 2012. Screening quantitative indictors of drought tolerance in bread wheat (Triticum aestivum L.) landraces. Eur J Exp Bio 2: 577-584.
Farshadfar E, Ghannadha M, Zahravi M, Sutka J. 2001. Genetic analysis of drought tolerance in wheat. Plant Breed 114: 542-544.
Farshadfar E, Poursiahbidi MM, Safavi SM. 2013. Assessment of drought tolerance in land races of bread wheat based on resistance/tolerance indices. Int J Adv Biol Biomed Res 1: 143-158.
Farshadfar E, Sutka J. 2002. Multivariate analysis of drought tolerance in wheat substitution lines. Cereal Res Commun 31: 33-40.
Fernandez GCJ. 1992. Effective selection criteria for assessing plant stress tolerance. Proceedings of the International Symposium on Adaptation of Vegetables and other Food Crops in Temperature and Water Stress, August 13-16, 1992, Shanhua, Taiwan, pp. 257-270.
Fischer RA, Wood JT. 1979. Drought resistance in spring wheat cultivars: III. Yield association with morpho-physiological traits. Aust J Agr Res 30: 1001-1020.
Gadimaliyeva G, Akparov Z, Aminov N, Aliyeva A, Ojaghi J, Salayeva S, Serpoush M, Mammadov A, Morgounov A. 2020. Assessment of synthetic wheat lines for soil salinity tolerance. Zemdirbyste 107: 55-62.
Gavuzzi P, Rizza F, Palumbo M, Campaline RG, Ricciardi GL, Borghi B. 1997. Evaluation of field and laboratory predictors of drought and heat tolerance in winter cereals. Can J Plant Sci 77: 523-531.
Ghasemi M, Farshadfar E. 2015. Screening drought tolerant genotypes in wheat using multivariate and stress tolerance score methods. Int J Biosci 6: 326-333.
Hooshmandi B. 2019. Evaluation of tolerance to drought stress in wheat genotypes. Idesia 37: 37-43.
Kamrani M, Hoseini Y, Ebadollahi A. 2018. Evaluation for heat stress tolerance in durum wheat genotypes using stress tolerance indices. Arch Agron Soil Sci 64: 38-45.
Khan AA, Kabir MR. 2014. Evaluation of spring wheat genotypes (Triticum aestivum L.) for heat stress tolerance using different stress tolerance indices. Cercet Agron Mold 160: 49-63.
Khan IM, Dhurve OP. 2016. Drought response indices for identification of drought tolerant genotypes in rainfed upland rice (Oryza sativa L.). Int J Sci Environ Technol 5: 73-83.
Khoshgoftar AH, Shariatmadari H, Karimian N. 2006. Responses of wheat genotypes to zinc fertilization under saline soil conditions. J Plant Nutr 29: 1543-1556.
Khoshgoftarmanesh AH, Razizadeh ES, Eshghizadeh HR, Savaghebi Gh, Sadrearhami A, Afuni D. 2011. Screening tolerance of different spring wheat genotypes to zinc deficiency with using different stress indices. J Water Soil 25: 287-295.
Khoshgoftarmanesh AH, Sadrarhami A, Sharifi HR, Afiuni D, Schulin R. 2009. Selecting zinc-efficient wheat genotypes with high grain yield using a stress tolerance index. Agron J 101: 1409-1416.
Lan J. 1998. Comparison of evaluating methods for agronomic drought resistance in crops. Acta Agr Bor-Occid Sinic 7: 85-87.
Lin CS, Binns MR, Lefkovitch LP. 1986. Stability analysis: Where do we stand? Crop Sci 26: 894-900.
Mitra J. 2001. Genetics and genetic improvement of drought resistance in crop plants. Curr Sci 80: 758-763.
Molla Heydari Bafghi R, Baghizadeh A, Mohammadinezhad G. 2017. Evaluation of salinity and drought stresses tolerance in wheat genotypes using tolerance indices. J Crop Breed 9: 27-34.
Moosavi SS, Yazdi-Samadi B, Naghavi MR, Zali AA, Dashti H, Pourshahbazi A. 2008. Introduction of new indices to identify relative drought tolerance and resistance in wheat genotypes. Desert 12: 165-178.
Pour-Siahbidi MM, Pour-Aboughadareh AR. 2013. Evaluation of grain yield and repeatability of drought tolerance indices for screening chickpea (Cicer aritinum L.) genotypes under rainfed conditions. Iranian J Genet Plant Breed 2(2): 28-37.
Rajaram S, Villareal R, Mujeeb-Kazi A. 1990. The Global Impact of 1B/1R Spring Wheats. Agronomy Abstracts, San Antono: American Society of Agronomy (ASA), Madison, USA. 105 p.‏
Royo C, Miloudi MM, Di Fonze N, Arraus JL, Pfeiffer WH, Slafer GA. 2005. Durum Wheat Breeding: Current Approaches and Future Strategies. Volumes 1 and 2. 1st Edition, CRC Press. 1112 p.
Saeidi M, Abdoli M, Shafiei-Abnavi M, Mohammadi M, Eskandari-Ghaleh Z. 2016. Evaluation of genetic diversity of bread and durum wheat genotypes based on agronomy traits and some morphological traits in non-stress and terminal drought stress conditions. Cereal Res 5: 353-369.
SAS Institute. 2011. Base SAS 9.1 Procedures Guide. SAS Institute Inc, Cary.
Shirani Rad AH, Abbasian A. 2011. Evaluation of drought tolerance in rapeseed genotypes under non stress and drought stress conditions. Not Bot Horti Agrobot Cluj Napoca 39: 164-171.
Sims JT, Johnson GV. 1991. Micronutrients Soil Tests. In: Mordcvedt JJ, Cox FR, Shuman LM, Welch RM. (eds.), Micronutrients in Agriculture. SSSA Book Series No. 4, Madison, WI. pp. 427-476.
SPSS. 2007. SPSS 16.0 for Windows. 16th Edition. New York, USA.
USDA. 2017. World Agriculture Production, United States Department of Agriculture. Available at: