Genetic Diversity of Bread Wheat (Triticum aestivum L.) Genotypes Using RAPD and ISSR Molecular Markers

Document Type : Research Article


1 Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran

2 Department of Molecular and Cell Biology, Marand Branch, Islamic Azad University, Marand, Iran

3 Department of Plant Biology, Marand Branch, Islamic Azad University, Marand, Iran


The importance of grain cultivation especially wheat is obvious in terms of providing human and animal food and its impact on the economy of human societies. The reduction of genetic diversity in cultivars prevents increasing yields in line with rising demand and consumption. Therefore, it is necessary to improve the compatibility of them and increase their genetic extent. In the current, the genetic diversity of Iranian wheat genotypes was investigated at the DNA level using RAPD and ISSR markers. 17 RAPD primers and 16 ISSR primers generated 86 (86/99= %86.86) and 56 (56/64= %57.5) polymorphic bands respectively. The cluster analysis based on UPGMA and dendrogram plotted using NTSYSpc 2.02 software revealed three main clusters. The highest genetic distance was between CD-89-2 and CD-89-7 genotypes and the minimum genetic distance was between CD-89-2 and CD-89-3 genotypes. Based on Nei's genetic distance matrix, the mean number of effective bands, the Shannon index, and polymorphism content were 1.381, 0.332, and % 87.12, respectively. Our results showed that RAPD and ISSR analysis are suitable methods to study genetic diversity and relationships among T. aestivum genotypes.


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