Evaluation of Long Non-Coding RNAs: HNF1A-AS1 and MVIH Expressions and Their Clinical Significance in Human Gastric Cancer

Document Type: Research Article

Authors

1 Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

2 Department of Genetics and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Child Growth and Development Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

4 Research Institute of Biotechnology, Shahrekord University, Shahrekord, Iran

10.22080/jgr.2020.18350.1173

Abstract

Gastric cancer is one of the most common cancers in the world. Late diagnosis is the main cause of the high rate of treatment failure and death among patients with gastric cancer; therefore identifying the molecular basis of cancer initiation and metastasis is so critical for developing efficient methods for early diagnosis and therapy. long non-coding RNAs (lncRNAs) are the largest group of noncoding RNAs, which are involved in cancer pathogenesis. Regarding the roles of lncRNA-HNF1A-AS1 and lncRNA-MVIH in cancer pathogenesis and progression, we aimed to evaluate expression profiles and clinicopathological relevance of these two genes in human gastric cancer. Real-time PCR was performed to assess relative gene expressions in 60 tumoral and non-tumoral gastric tissues. The association between gene expressions and clinicopathological characteristics were also analyzed. Expression and clinicopathological data of the lncRNA-HNF1A-AS1 from 318 gastric cancer patients were also downloaded from the TCGA database. HNF1A-AS1 was down-regulated in GC tissues and MVIH did not show any significant differential expression in GC tissues. Otherwise, the expression of lncRNA-HNF1A-AS1 was significantly higher in TCGA tumor samples. Furthermore, lncRNA-HNF1A-AS1 expression was associated with tumor metastasis and that of lncRNA-MVIH showed association with tumor grade and stage. lncRNA-HNF1A-AS1 expression status did not show any significant correlation with GC overall survival. In conclusion, lncRNA-HNF1A-S1 and lncRNA-MVIH genes may play a critical role in gastric cancer progression. Functional studies on the mechanism of action of these two lncRNAs could help to understand their role in gastric cancer progression.

Keywords


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